讲座题目：PLANT IMMUNOLOGY: CRACKING THE REDOX CODE

讲座人：Prof. Gary J. Loake（Institute of Molecular Plant Science, University of Edinburgh, Edinburgh, UK）

讲座时间：2012年9月12日（星期三）上午9：30

讲座地点：林学院学术报告厅(主西517室)

内容简介：

Changes in redox status are a conspicuous feature of immune responses in a variety of eukaryotes, but the associated signalling mechanisms are not well understood. In plants, attempted microbial infection triggers the rapid synthesis of nitric oxide (NO) and a parallel accumulation of reactive oxygen intermediates (ROIs), the latter of which is generated by NADPH oxidases related to those responsible for the pathogen-activated respiratory burst in phagocytes. Both NO and ROIs have been implicated in immune signalling and the control of the hypersensitive response (HR), a programmed execution of plant cells at sites of attempted infection. Our findings suggest that S-nitrosylation, the addition of an NO moiety to a protein cysteine thiol to form an S-nitrosothiol, is a key regulator of the plant defence response, controlling ROI synthesis, the accumulation of the immune activator, salicylic acid (SA) and cognate SA signalling. We are employing a variety of complementary approaches, including: forward and reverse genetics, Solexa-based gene expression profiling and novel proteomics strategies, to uncover the molecular landscape of S-nitrosylation during plant immune function.

Relevant Publications

Yun, B-W., Feechan, A., Yin, M., Saidi, N. B.B., Yu, M., Le Bihan, T. Kang, J.-G., Kwon, E., Spoel, S., Pallas, J.A. and Loake, G.J. (2011). S-nitrosylation of NADPH oxidase regulates cell death in plant immunity. Nature 478, 264-268.

Feechan, A., Kwon, E.-J., Yun, B.-W., Wang, Y., Pallas, J.A. and Loake, G.J. (2005).A Central Role for S-Nitrosothiols in Plant Disease Resistance.Proc. Natl. Acad. Sci. USA 102, 8054-8059.

Moore, J., Loake, G.J. and Spoel, S.H. (2011).Transcription dynamics in plant immunity. Plant Cell 23, 2809-2820.

Wang, Y.-Q., Feechan, A., Yun, B.-W., Shafiei, R., Hofmann, A., Taylor, P., Xue, P., Yang, F.-Q., Xie, Z.-S., Pallas, J.A., Chu, C.-C. andLoake, G.J. (2009). S-nitrosylation of AtSABP3 antagonises the expression of plant immunity. J. Biol. Chem. 284, 2131-2137.

Spoel, S.H. and Loake, G.J. (2011). Redox-based protein modifications: the missing link in plant immune signalling. Current Opinion Plant Biology 14, 358-364.

Yu, M., Yun, B.-W.Spoel, S.H. and Loake, G.J. (2012). A sleigh ride through the SNO: regulation of plant immune function by S-nitrosylation. Current Opinion in Plant Biology 15, 424-431.